Chemically defined DMEM

Chemically Defined Dulbecco’s Modified Eagle’s Medium (CD-DMEM)

(18 customer reviews)

 

 

SKU: P3A210105 Categories: Cell Culture Media, Dulbecco’s Modified Eagle’s Medium (DMEM) Tags: Advanced, Cell Culture, DMEM, Dulbecco's, Human Transferrin, L-Alanyl-L-Glutamine, Recombinant Insulin, Serum Reduction

Description

Background:

Over the past three decades, PurMa Biologics has tenaciously worked to develop fully synthetic cell culture media by eliminating any animal products. We are proud to have developed and over the years fully evaluated every aspect of Chemically Defined forms of all the conventional media such as DMEM, DMEM/F12, IMDM, RPMI-1640 and…

PurMa TM Chemically Defined DMEM is a proprietary formulated used to grow cells invitro without any undefined or animal-derived components. CD-DMEM contains known quantities of pure chemicals, including amino acids, vitamins, salts, sugars, and more importantly a proprietary combination of synthetic growth factors. This provides a consistent and controlled environment for cell growth and research. Noticeably, CD-DMEM is essential for applications requiring reproducibility, regulatory compliance. Moreover, for studying specific cellular processes. without extraneous variables. Furthermore, CD-DMEM is designed for custom formulations tailored for various cell types.

Chemically Defined DMEM Vs 10% FBS

Special Features:

  • The results from PurMaTM CD-DMEM are consistent, reproducible and expect personal errors can be very similar between labs across the world.
  • Evidently, based on the graph below, PurMaTM CD DMEM is completely comparable with standard DMEM+ %10 FBS
  • It Contains a proprietary combination of synthetic growth factors, synthetic hormones synthetic proteins and chemicals.
  • Most importantly, PurMaTM CD DMEM supports the full growth of primary and commercial cell lines.
  • Also, we use L-Alanine- L-Glutamine in all or chemically defined media.
  • Moreover, antibiotics can be added as regular to PurMaTM CD-DMEM

Formulation: Complete formulation is available here: Proprietary

References:

  1. Development of a Chemically Defined Medium for in vitro Expansion of Primary Bovine Satellite Cells. Kolkmann AM. et al, 2022, 4;10:895289
  2. CNTF+BDNF treatment and neuroprotective pathways in the rd1 mouse retina. Azadi, S. et al, 2007, 19;1129(1):116-29

 

 

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.1 µm filter
Form Liquid
Shipping Condition  Room temperature, overnight

 

Additional information

Condition

A-DMEM – Standard Formulation, A-DMEM – Standard Formulation, ATCC Formulation, A-DMEM – Without L-Alanyl-L-Glutamine, A-DMEM – Without Phenol Red, A-DMEM – Without Sodium Bicarbonate, A-DMEM – With Sodium Pyruvate, A-DMEM – Without Glucose, A-DMEM – With Lipoic Acid, A-DMEM – Without L-Alanyl-L-Glutamine, Without Phenol Red, A-DMEM – Without L-Alanyl-L-Glutamine, Without Sodium Bicarbonate, A-DMEM – Without L-Alanyl-L-Glutamine, Without Phenol Red, Without Sodium Bicarbonate, A-DMEM – Low Glucose (1.5 g/L), A-DMEM – Low Sodium Bicarbonate (1.5 g/L), A-DMEM – 15 mM HEPES (3.6 g/L), A-DMEM – 25 mM HEPES (5.9 g/L), A-DMEM – 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM – 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), A-DMEM – Without Phenol Red, Without Sodium Bicarbonate, A-DMEM – Without phenol Red, 15 mM HEPES (3.6 g/L), A-DMEM – Without phenol Red, 25 mM HEPES (5.9 g/L), A-DMEM – Without phenol Red, Low Sodium Bicarbonate (1.5 g/L)
Format

Liquid
Size

1 x 100 ml, 1 x 500 ml, 1 x 1000 ml

18 reviews for Chemically Defined Dulbecco’s Modified Eagle’s Medium (CD-DMEM)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

  12. hyeungk1968@gmail.comthe –

    Review: I used Lipid Free horse serum of PurMa Biologics.
    The quality is great!
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 12:09:58
    Ease of placing the order: 5/5

  13. jaya-krishnan@omrf.org –

    Review: I started my lab using the regular Gibco media components. We have shifted to Purma past 6 months and see no difference in the quality of our cell culture. Simply saying that Purma is cheaper, quicker to deliver and as good as Gibco. The best part was that Purma customized several media for our fish cell lines which was really helpful. Lastly, Seif walked through the customization of media over Zoom, that gave a lot of confidence for using those media solutions.
    Have you purchased: Yes
    Time of submission: 17-Mar-2025 14:22:58
    Ease of placing the order: 4/5

  14. tayyab-afzal@omrf.org –

    Review: We bought Premium FBS and DMEM cell culture media from PurMa Biologics. We are using them for several mammalian cell line and primary cell culture and the cell growth is perfectly fine. We are totally satisfied with the product quality. Importantly as local vendor, we can have the product available as per our demand ASAP.
    Have you purchased: Yes
    Time of submission: 18-Mar-2025 10:01:50
    Ease of placing the order: 5/5

  15. Fitzkr@gmail.com –

    Review: We purchase everything we need related to cell and tissue culture from PurMa, reagents like: DMEM, LL15, EMEM, FBS, trypsin, antibiotics mix100X and…
    Always great quality, fast processing and shipping.
    We are totally satisfied.
    Have you purchased: Yes
    Time of submission: 22-Mar-2025 10:47:00
    Ease of placing the order: 5/5

  16. vmadka@ouhsc.edu –

    Review:
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:02:40
    Ease of placing the order: 4/5

  17. Anupam-Mandal@ouhsc.edu –

    Review: Excellent
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:05:43
    Ease of placing the order: 5/5

  18. rohan-varshney@ouhsc.edu –

    Review: Reliable and dependable products. We purchased lipid-depleted FBS (LD-FBS) from PurMa Biologics and it worked great. We cross-checked the lipid content in the serum by GC-MS and Triglyceride assay and as advertised, did not find any lipids in the FBS.
    Have you purchased: Yes
    Time of submission: 24-Mar-2025 15:06:38
    Ease of placing the order: 5/5

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